Sandra Clifton, Ph.D.
Genome Sequencing Center
Washington Univ. School of Medicine
4444 Forest Park Blvd.
St. Louis, Missouri 63108
E-mail: sclifton@watson.wustl.edu |
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CURRENT ACADEMIC APPOINTMENTS
Research Instructor, 1998 -Present
Group Leader of Darwin Expressed Sequence Tag
(EST) Projects, 1999-Present-Currently lead the sequencing effort
has supplied ESTs from human and mouse, and is now supplying ESTs
from soybean, endocrine pancreas, Eimeria,, Neospora, Plasmodium,
moss, 18 parasitic nematode species, Sarcocystis, Toxoplasma,
Xenopus, and zebrafish.
Funds for producing hydra ESTs have just been awarded (10/01).
(link to EST page)
Group Leader of Small Projects-1998-Present-Directed
the sequencing, finishing and annotation of Salmonella enterica
var Typhimurium, 8X whole genome shotgun coverage of Klebsiella
pneumoniae, along with survey sequencing (4X whole genome shotgun
coverage of Salmonella enterica var Paratyphi A. The paper reporting
these results will soon be published in the journal Nature. (link
when paper comes out) Funds for sequencing, finishing and annotating
2 maize mitochondrial genomes, along with sample sequencing of
3 other maize mitochondrial genomes and an outlier sorghum mitochondrial
genome have just been awarded (9/01).
SELECTED PUBLICATIONS
Clifton, S.W., McCarthy, D., Roe, B.A. (1994)
Sequence of the rec-2 Locus of Haemophilus influenzae: Homologies
to comE-ORF3 of Bacillus subtilis and msbA of Escherichia coli.
Gene 146, 95-100
Toal, D. R., Clifton, S. W., Roe, B. A., Downard,
J. (1995) The esg Locus of Myxococcus xanthus encodes the E1a
and E1b Subunits of a Branched-chain Keto Acid Dehydrogenase.
Mol. Microbiol. 16, 177-189.
Chissoe, S. L., Bodenteich, A., Wang, Y. F.,
Wang, Y. P., Burian, D., Clifton, S. W., Crabtree, J., Freeman,
A., Iyer, K., Jian, L., Ma, Y., McLaury, H. J., Pan, H. Q., Sarhan,
S., Toth, S., Wang, Z., Zhang, G., Heisterkamp, N., Groffen, J.,
Roe, B.A. (1995) Sequence and Analysis of the Human ABL Gene,
the BCR Gene, and Regions Involved in the Philadelphia Chromosomal
Translocation. Genomics 27, 67-82.
Glusman G., Clifton, S., Roe, B., Lancet, D.
(1996) Sequence Analysis in the Olfactory Receptor Gene Cluster
on Human Chromosome 17: Events Affecting Receptor Diversity. Genomics
37, 147-160.
Guilbaud, C., Peyard, M., Fransson, I., Clifton,
S. W., Roe, B. A., Carter, J. D.
Dumanski, J. P. (1997) Characterization of the Mouse b-prime Adaptin
Gene; cDNA sequence, genomic structure, and chromosomal localization.
Mamm Genome 8, 651-656.
Akopyants, N. S., Clifton, S. W., Kersulyte,
D., Crabtree, J. E., Youree, B. E.,
Reece, C. A., Bukanov, N. O., Drazek, E. S., Roe, B. A., Berg,
D. E. (1998) Analyses of the cag Pathogenicity Island of Helicobacter
pylori. Mol. Microbiol. 28, 37-53.
Glusman, G., Sharon, D., Kalash, F., Clifton,
S., Roe, B., Ferraz, C., Demaille, J., Ben-Asher, E., Lancet,
D. (1999). Genome Dynamics, Polymorphisms and Mutations in a 350
Kb Human Olfactory Receptor Gene Cluster. Mathematical Modelling
and Scientific Computing 9 (1), 30-44.
Marra, M., Hillier, L., Kucaba,T., Allen, M,
Barstead, R., Beck, C., Blistain, A. Bonaldo, M., Bowers, Y.,
Bowles, L., Cardenas, M., Chamberlain, A., Chappell, J., Clifton,
S., Favello, A., Geisel, S., Gibbons, M., Harvey, N., Hill, F.,
Jackson, Y., Kohn, S., Lennon, G., Mardis, E., Martin, J., Mila,
L., McCann, R., Morales, R., Pape, D., Person, B., Prange, C.,
Ritter, E., Soares, M., Schurk, R., Shin, T., Steptoe, M., Swaller,
T., Theising, B., Underwood, K., Wylie, T., Yount, T., Wilson,
R., Waterston, R. (1999) An Encyclopedia of Mouse Genes. Nature
Genetics 21, 191-194
McClelland, M., Florea, L., Sanderson, K., Clifton,
S. W., Wilson, R. K., Miller, W. (2000) Comparison of the E. coli
K12 Genome with Sampled Genomes of a Klebsiella pneumoniae and
three Salmonella enterica serovars., Typhimurium, Typhi, and Paratyphi.
Nucleic Acids Res., 28, 4974-4986.
McCarter, J.P., Bird, D. McK., Clifton, S. W.,
Waterston, R. H. (2000) Nematode Gene Sequences, December, 2000
Update. Journal of Nematology, 32, 331-333.
Ferretti, J.J., McShan, W.M., Ajdic, D., Savic,
D.J., Savic, G., Lyon, K., Primeaux, C., Sezate, S., Suvarov,
A.N., Kenton, S., Lai, H-S., Lin, S-P., Qian, Y., Jia, H.G., Najar,
F.Z., Ren, Q., Zhu, H., Song, L., White, J., Yuan, X., Clifton,
S.W., Roe, B.A., McLaughlin, R. (2001) Complete genome sequence
of an M1 strain of Streptococcus pyogenes. Proc Natl
Acad Sci U S A. 98, 4658-63.
The Genome International Sequencing Consortium
(2001) Initial Sequencing and Analysis of the Human Genome. Nature
409, 860-921.
Clark MD, Hennig S, Herwig R, Clifton SW, Marra
MA, Lehrach H, Johnson SL, Group T. W. (2001) An Oligonucleotide
Fingerprint Normalized and Expressed Sequence Tag Characterized
Zebrafish cDNA Library. Genome Res. 9,1594-1602
McClelland, M., Sanderson, K E., Spieth, J.,
Clifton, S. W., Latreille, P., Courtney, L., Ali, J., Dante, M.,
Du, F., Hou, S., Layman, D., Leonard, S., Nguyrn, C., Scott, K.,
Holmes, A., Grewal, N., Mulvaney, E., Ryan, E., Sun, H., Florea,
L., Miller, W., Porwollik, S., Stoneking, T., Nhan, M., Waterston,
R., Wilson, R. K. (2001) The Complete Genome Sequence of Salmonella
enterica serovar Typhimurium LT2: Features Revealed by Comparison
to Related Genomes. (Manuscript in review)
.Johnson, S. L., Waterman, R. E., Noriega-Fenton,
L., Sheng, X., Hersey, C., Rauch, G.-J., Geisler, R., Fischer,
D., Barbazuk, B., Cox, K., Ekker, M., Hukriede, N., Daniluk, D.,
Song, A., Arodi, S., Foote, H., Sugano, S., Kawakami, K., Wu.
N., Brockerhoff, S., Lee, R., Ngai, J., Clark, M., Prange, C.,
Zon, L,, Clifton, S., McvPherson, J. D., and Zhou, Yi (2001) Integrated
Zebrafish Transcript Map and Comparison to the Human Genome. (Manuscript
in review)
